Journal: International Journal of Molecular Sciences
Article Title: Targeting NF-κB by the Cell-Permeable NEMO-Binding Domain Peptide Improves Albuminuria and Renal Lesions in an Experimental Model of Type 2 Diabetic Nephropathy
doi: 10.3390/ijms21124225
Figure Lengend Snippet: Cell-permeable NBD peptide decreased podocyte loss and basement membrane thickness in BTBR ob/ob mouse kidney. ( A ) Representative images of WT-1 immunohistochemistry (magnification 630×) and quantification of WT-1 + cells in glomerular cross section (gcs). Transmission electron microscopy images (magnification 9900×) and measurement of ( B ) glomerular basement membrane (GBM) and ( C ) tubular basement membrane (TBM) thickness. Data shown as scatter dot plots and mean ± SD of each group (n = 6–8 mice/group). *** p < 0.001; **** p < 0.0001 vs. diabetic vehicle control.
Article Snippet: The primary antibodies for immunodetection studies were: WT-1 podocyte marker (Agilent Cat# M3561, RRID:AB_2304486, dilution 1:100, Santa Clara, CA, USA), F4/80 monocytes/macrophages (Bio-Rad Cat# MCA497, RRID:AB_2098196, dilution 1:70, Hercules, CA, USA), CD3 T lymphocytes (Agilent Cat# M7254, RRID:AB_2631163, dilution 1:100Santa Clara, CA, USA), p -p65 serine 536 (Santa Cruz Biotechnology Cat# sc-33020, RRID:AB_2179018, dilution 1:100, Santa Cruz, CA, USA), p -STAT3 tyrosine 705 (Cell Signaling Technology Cat# 9134, RRID:AB_331589, dilution 1:100, Leiden, The Netherlands) and p -Nrf2 serine 40 (Abcam Cat# ab76026, RRID:AB_1524049, dilution 1:2000, Cambridge, UK).
Techniques: Immunohistochemistry, Transmission Assay, Electron Microscopy